Overview
- Product nameEpiSeeker HDAC1 Sumoylation Assay Kit
- Sample typeCell culture extracts, Tissue Extracts
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: all Mammals - Product overview
Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners.
HDAC1 is a class I histone deacetylase that plays a critical role in transcriptional repression of gene expression and has been shown to be an integral component of multiprotein co-repressor complexes. It has been observed that an important mechanism of HDAC1 activity regulation is sumoylation, which acts by potentiating HDAC1 activity.
EpiSeeker HDAC1 Sumoylation Assay Kit (ab115132) allows the user to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues extracts.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units 8-Well Control Strips 2 units 3 units ESH1 (10X Wash Buffer) 1 x 25ml 1 x 50ml ESH2 (Sumo Assay Buffer) 1 x 2ml 1 x 4ml ESH3 (Sumo Protein, 1 µg/µl) 1 x 6µl 1 x 12µl ESH4 (Sumo Antibody, 1µg/µl 1 x 5µl 1 x 10µl ESH5 (Signal Report Solution) 1 x 10µl 1 x 20µl ESH6 (Color Development Solution) 1 x 6ml 1 x 12ml ESH7 (Stop Solution) 1 x 3ml 1 x 6ml Signal Enhancer * 1 x 120µl 1 x 240µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker HDAC1 Sumoylation Assay Kit (ab115132)
ab115132 has not yet been referenced specifically in any publications.
