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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners.
HDAC1 is a class I histone deacetylase that plays a critical role in transcriptional repression of gene expression and has been shown to be an integral component of multiprotein co-repressor complexes. It has been observed that an important mechanism of HDAC1 activity regulation is sumoylation, which acts by potentiating HDAC1 activity.
EpiSeeker HDAC1 Sumoylation Assay Kit (ab115132) allows the user to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues extracts.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|8-Well Control Strips||2 units||3 units|
|ESH1 (10X Wash Buffer)||1 x 25ml||1 x 50ml|
|ESH2 (Sumo Assay Buffer)||1 x 2ml||1 x 4ml|
|ESH3 (Sumo Protein, 1 µg/µl)||1 x 6µl||1 x 12µl|
|ESH4 (Sumo Antibody, 1µg/µl||1 x 5µl||1 x 10µl|
|ESH5 (Signal Report Solution)||1 x 10µl||1 x 20µl|
|ESH6 (Color Development Solution)||1 x 6ml||1 x 12ml|
|ESH7 (Stop Solution)||1 x 3ml||1 x 6ml|
|Signal Enhancer *||1 x 120µl||1 x 240µl|
Our Abpromise guarantee covers the use of ab115132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115132 has not yet been referenced specifically in any publications.
Publishing research using ab115132 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"