Overview

  • Product nameEpiSeeker HDAC1 Sumoylation Assay Kit
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: all Mammals
  • Product overview

    Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners.

    HDAC1 is a class I histone deacetylase that plays a critical role in transcriptional repression of gene expression and has been shown to be an integral component of multiprotein co-repressor complexes. It has been observed that an important mechanism of HDAC1 activity regulation is sumoylation, which acts by potentiating HDAC1 activity.

    EpiSeeker HDAC1 Sumoylation Assay Kit (ab115132) allows the user to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues extracts.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115132 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker HDAC1 Sumoylation Assay Kit (ab115132)

    ab115132 has not yet been referenced specifically in any publications.

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