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EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)

Q&A (1)

Overview

  • Product nameEpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay KitSee all KDM1 / LSD1 kits ...
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human, Plants
    Predicted to work with: all Mammals, Other
  • Product overview

    KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.

    EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) allows the user to measure activity/inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

  • Notes

    Store EKD3, EKD4, and EKD6, at –20°C away from light.

    Store all other components (EKD1, EKD2, EKD5, EKD7, and 8-Well Assay Strip) at 4°C away from light.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
    • Alternative names
        Amine oxidase (flavin containing) domain 2AOF2BHC110
        BRAF35-HDAC complex protein BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunitFlavin-containing amine oxidase domain-containing protein 2KDM1KIAA0601LSD1Lysine (K) specific demethylase 1Lysine (K) specific demethylase 1ALysine-specific histone demethylase 1Lysine-specific histone demethylase 1A
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab113457 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Functional Studies FuncS

      EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit images

      • Functional Studies - EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
        Functional Studies - EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
        LSD1 activity measurement with kit ab113457. Recombinant human LSD1 enzyme was added at different concentrations.
        Image A: Original RFU data. RFU is inversely proportional to LSD1 enzyme activity.
      • Functional Studies - EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
        Functional Studies - EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
        Image B: B: RFU data after conversion from A.
      • Functional Studies - EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
        Functional Studies - EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
        LSD1 activity measured from NIH-3T3 cell extracts.

      Protocols

      References for EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)

      ab113457 has not yet been referenced specifically in any publications.

      Publishing research using ab113457 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab113457 Submit a Question for ab113457

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      I am working for Dana Farber Cancer Institute, Boston, MA.Our labis looking for a ELISA kit which can measure the activity of LSD1.For this, we are trying to understand the difference between all the 4 ELISA kits for LSD1(named below) ABcam is providing. ...

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      Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a color...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"