EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)

Overview

  • Product nameEpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay KitSee all KDM1 / LSD1 kits ...
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human, Plants
    Predicted to work with: all Mammals, Other
  • Product overview

    KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.

    EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) allows the user to measure activity/inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

  • Notes

    Store EKD3, EKD4, and EKD6, at –20°C away from light.

    Store all other components (EKD1, EKD2, EKD5, EKD7, and 8-Well Assay Strip) at 4°C away from light.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
  • Alternative names
    • Amine oxidase (flavin containing) domain 2
    • AOF2
    • BHC110
    • BRAF35-HDAC complex protein BHC110
    • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
    • Flavin-containing amine oxidase domain-containing protein 2
    • KDM1
    • KIAA0601
    • LSD1
    • Lysine (K) specific demethylase 1
    • Lysine (K) specific demethylase 1A
    • Lysine-specific histone demethylase 1
    • Lysine-specific histone demethylase 1A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit images

  • LSD1 activity measurement with kit ab113457. Recombinant human LSD1 enzyme was added at different concentrations.
    Image A: Original RFU data. RFU is inversely proportional to LSD1 enzyme activity.
  • Image B: B: RFU data after conversion from A.
  • LSD1 activity measured from NIH-3T3 cell extracts.

Protocols

References for EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)

ab113457 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"