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KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) allows the user to measure activity/inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
Store EKD3, EKD4, and EKD6, at –20°C away from light.
Store all other components (EKD1, EKD2, EKD5, EKD7, and 8-Well Assay Strip) at 4°C away from light.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|EKD1 (10X Wash Buffer)||1 x 15ml||1 x 30ml|
|EKD2 (LSD1 Assay Buffer)||1 x 2ml||1 x 4ml|
|EKD3 (LSD1 Substrate)||1 x 50µl||1 x 100µl|
|EKD4 (LSD1 Assay Standard, 20 µg/ml||1 x 25µl||1 x 50µl|
|EKD5 (Capture Antibody, 1000 µg/ml)||1 x 5µl||1 x 10µl|
|EKD6 (Detection Antibody, 200 µg/ml)||1 x 8µl||1 x 16µl|
|EKD7 (Fluoro-Developer)||1 x 6ml||1 x 12µl|
Our Abpromise guarantee covers the use of ab113457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113457 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"