EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
- Product nameEpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay KitSee all KDM1 / LSD1 kits ...
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human, Plants
Predicted to work with: all Mammals, Other
- Product overview
KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) allows the user to measure activity/inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
Store EKD3, EKD4, and EKD6, at –20°C away from light.
Store all other components (EKD1, EKD2, EKD5, EKD7, and 8-Well Assay Strip) at 4°C away from light.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units EKD1 (10X Wash Buffer) 1 x 15ml 1 x 30ml EKD2 (LSD1 Assay Buffer) 1 x 2ml 1 x 4ml EKD3 (LSD1 Substrate) 1 x 50µl 1 x 100µl EKD4 (LSD1 Assay Standard, 20 µg/ml 1 x 25µl 1 x 50µl EKD5 (Capture Antibody, 1000 µg/ml) 1 x 5µl 1 x 10µl EKD6 (Detection Antibody, 200 µg/ml) 1 x 8µl 1 x 16µl EKD7 (Fluoro-Developer) 1 x 6ml 1 x 12µl
- Research Areas
- Amine oxidase (flavin containing) domain 2AOF2BHC110
- BRAF35-HDAC complex protein BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunitFlavin-containing amine oxidase domain-containing protein 2KDM1KIAA0601LSD1Lysine (K) specific demethylase 1Lysine (K) specific demethylase 1ALysine-specific histone demethylase 1Lysine-specific histone demethylase 1A
Our Abpromise guarantee covers the use of ab113457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit images
LSD1 activity measurement with kit ab113457. Recombinant human LSD1 enzyme was added at different concentrations.
Image A: Original RFU data. RFU is inversely proportional to LSD1 enzyme activity.
Image B: B: RFU data after conversion from A.
LSD1 activity measured from NIH-3T3 cell extracts.
References for EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
ab113457 has not yet been referenced specifically in any publications.