EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
- Product nameEpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay KitSee all KDM1 / LSD1 kits ...
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human, Plants
Predicted to work with: all Mammals, Other
- Product overview
KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) allows the user to measure activity/inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
Store EKD3, EKD4, and EKD6, at –20°C away from light.
Store all other components (EKD1, EKD2, EKD5, EKD7, and 8-Well Assay Strip) at 4°C away from light.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units EKD1 (10X Wash Buffer) 1 x 15ml 1 x 30ml EKD2 (LSD1 Assay Buffer) 1 x 2ml 1 x 4ml EKD3 (LSD1 Substrate) 1 x 50µl 1 x 100µl EKD4 (LSD1 Assay Standard, 20 µg/ml 1 x 25µl 1 x 50µl EKD5 (Capture Antibody, 1000 µg/ml) 1 x 5µl 1 x 10µl EKD6 (Detection Antibody, 200 µg/ml) 1 x 8µl 1 x 16µl EKD7 (Fluoro-Developer) 1 x 6ml 1 x 12µl
- Research Areas
- RelevanceHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
- Amine oxidase (flavin containing) domain 2
- BRAF35-HDAC complex protein BHC110
- FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
- Flavin-containing amine oxidase domain-containing protein 2
- Lysine (K) specific demethylase 1
- Lysine (K) specific demethylase 1A
- Lysine-specific histone demethylase 1
- Lysine-specific histone demethylase 1A
Our Abpromise guarantee covers the use of ab113457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit images
LSD1 activity measurement with kit ab113457. Recombinant human LSD1 enzyme was added at different concentrations.
Image A: Original RFU data. RFU is inversely proportional to LSD1 enzyme activity.
Image B: B: RFU data after conversion from A.
LSD1 activity measured from NIH-3T3 cell extracts.
References for EpiSeeker Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)
ab113457 has not yet been referenced specifically in any publications.