EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456)
- Product nameEpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay KitSee all KDM1 / LSD1 kits ...
- Assay time1h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456) allows the user to measure inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for screening KDM1/LSD1 inhibitors, which are directly interacting with he enzyme or blocking the binding to its substrate.
Store EKD2 and EKD5 at –20°C away from light.
Store remaining components (EKD1, EKD3, EKD4, EKD6, EKD7, and 8-Well Assay Strips) at 4°C away from light.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units EKD1 (LSD1 Assay Buffer) 1 x 2ml 1 x 4ml EKD2 (LSD1 Substrate, 0.7 mM) 1 x 150µl 1 x 300µl EKD3 (LSD1 Assay Standard, 100 mM) 1 x 10µl 1 x 20µl EKD4 (LSD1 Inhibitor, 1 mM) 1 x 25µl 1 x 50µl EKD5 (Fluoro Developer) 1 x 12µl 1 x 24µl EKD6 (Fluoro Enhancer) 1 x 12µl 1 x 24µl EKD7 (Fluoro Diluter) 1 x 4ml 1 x 8ml
- Research Areas
- Amine oxidase (flavin containing) domain 2AOF2BHC110
- BRAF35-HDAC complex protein BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunitFlavin-containing amine oxidase domain-containing protein 2KDM1KIAA0601LSD1Lysine (K) specific demethylase 1Lysine (K) specific demethylase 1ALysine-specific histone demethylase 1Lysine-specific histone demethylase 1A
Our Abpromise guarantee covers the use of ab113456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit images
Inhibition of KDM1/LSD1 by tranylcypromine (TCP) detected using ab113456. [LSD1 concentration: 500 ng/well].
References for EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456)
ab113456 has not yet been referenced specifically in any publications.