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KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
EpiSeeker Histone Demethylase KDM1/LSD1 Inhibitor Assay Kit (ab113456) allows the user to measure inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for screening KDM1/LSD1 inhibitors, which are directly interacting with he enzyme or blocking the binding to its substrate.
Store EKD2 and EKD5 at –20°C away from light.
Store remaining components (EKD1, EKD3, EKD4, EKD6, EKD7, and 8-Well Assay Strips) at 4°C away from light.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|EKD1 (LSD1 Assay Buffer)||1 x 2ml||1 x 4ml|
|EKD2 (LSD1 Substrate, 0.7 mM)||1 x 150µl||1 x 300µl|
|EKD3 (LSD1 Assay Standard, 100 mM)||1 x 10µl||1 x 20µl|
|EKD4 (LSD1 Inhibitor, 1 mM)||1 x 25µl||1 x 50µl|
|EKD5 (Fluoro Developer)||1 x 12µl||1 x 24µl|
|EKD6 (Fluoro Enhancer)||1 x 12µl||1 x 24µl|
|EKD7 (Fluoro Diluter)||1 x 4ml||1 x 8ml|
Our Abpromise guarantee covers the use of ab113456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113456 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"