EpiSeeker Histone Extraction Kit (ab113476)
- Product nameEpiSeeker Histone Extraction Kit
- Tests1 x 100 test
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time1h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
EpiSeeker Histone Extraction Kit (ab113476) provides a simple and selective method for extracting histone proteins in just 1 hour for a variety of applications such as histone modification such as acetylation, methylation, and sumoylation. ab113476 can be used to extract histones from as low as 105 cells or 1 mg of tissue.
Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.
Store kit at 4°C. Check if the buffers contain salt precipitates before use. If so, warm (at room temperature or 37°C) and shake the buffers until the salts are re-dissolved.
- Tested applicationsFunctional Studies more details
Components 100 tests 10X Pre-Lysis Buffer 1 x 10ml Balance Buffer 1 x 8ml DTT Solution 1 x 20µl Lysis Buffer 1 x 20ml
- Research Areas
Our Abpromise guarantee covers the use of ab113476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS. Total histone protein yield = 0.4mg/107 or 100mg tissue (yield may vary depending on cell or tissue type).|
EpiSeeker Histone Extraction Kit images
Histones were purified from mouse liver cells using ab113476. Each lane was loaded with 15μg of purified histone.
Image courtesy of Kevin Dalgaard, MPI for Immunobiology & Epigenetics, Freiburg, DE
Histone extracts were prepared from MCF-7 cells using the ab113476 and acetyl histone H3-K9 was quantified.
References for EpiSeeker Histone Extraction Kit (ab113476)
ab113476 has not yet been referenced specifically in any publications.