Overview

  • Product nameEpiSeeker Histone Extraction Kit
  • Tests
    1 x 100 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    EpiSeeker Histone Extraction Kit (ab113476) provides a simple and selective method for extracting histone proteins in just 1 hour for a variety of applications such as histone modification such as acetylation, methylation, and sumoylation. ab113476 can be used to extract histones from as low as 105 cells or 1 mg of tissue.

    Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.

  • Notes

    Store kit at 4°C. Check if the buffers contain salt precipitates before use. If so, warm (at room temperature or 37°C) and shake the buffers until the salts are re-dissolved.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab113476 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration. Total histone protein yield = 0.4mg/107 or 100mg tissue (yield may vary depending on cell or tissue type).

    EpiSeeker Histone Extraction Kit images

    • Histones were purified from mouse liver cells using ab113476. Each lane was loaded with 15μg of purified histone.

      Image courtesy of Kevin Dalgaard, MPI for Immunobiology & Epigenetics, Freiburg, DE

    • Histone extracts were prepared from MCF-7 cells using the ab113476 and acetyl histone H3-K9 was quantified.

    Protocols

    References for EpiSeeker Histone Extraction Kit (ab113476)

    ab113476 has not yet been referenced specifically in any publications.

    Product Wall

    The equivalent G Force values are:

    1000 RPM = 110G
    3000 RPM = 1000G
    10,000 RPM = 8000G
    12,000 RPM = 11,000G

    No special preparation such as desalting needs to be done before loading the sample into the gel.

    ab113476 does not contain any HDAC inhibitors.

    The equivalent G force values are: 1000 RPM = 110G 3000 RPM = 1000G 10,000 RPM = 8000G 12,000 RPM = 11,000G

    I can confirm that ab113476: EpiSeeker Histone Extraction Kit would be suitable to use for human blood and skin and would extract total histone proteins, including H1, that would be quantifiable.

    Nuclear Protein extraction and Histone protein extraction from cell samples frozen at -80oC could still work using ab113474 and ab113476. However, the enzyme activity of nuclear extracts from frozen tissues may be much lower than that from fresh tissues.

    Ab113476 can be used to extract histones from a protozoan parasite if you use the protocol for histone extraction from tissues.

    yes ab113476 can be used for extracting histone proteins from frozen whole blood cells. For the best results, the amount of leukocyte cells in the whole blood should be estimated so that proper volume of whole blood can be used for extracting sufficien...

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    Hello! The two kits ab115102 and ab113476 should be suitable for use in fish, while ab133099 is designed to study inhibitors and so fish is not suitable. Please let me know if you have further questions.

    The extract prepared by this kit is suitable for WB analysis. There is no special preparation or treatment needed for loading the extract sample to the gel. Lysis buffer does not need to be added to extract the protein. Please use common protocol as us...

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    Thank you for contacting Abcam.

    We recently spoke on the phone regarding a Histone H3 methylation study. Below I have summarized our conversation and answers to your questions.

    For detecting nuclear proteins via western blot we recomm...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"