Overview
- Product nameEpiSeeker Histone H3 Acetylation Assay KitSee all Histone H3 acetylation kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.
EpiSeeker Histone H3 Acetylation Assay Kit (ab115102) allows the user to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
- Notes
Store the Acetylated Histone H3 Control and EAH8 at –20°C away from light.
Store EAH3, EAH5, EAH6, EAH7, EAH9, and the 8-Well Assay Strips at 4°C away from light.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units Acetylated Histone H3 Control (60 µg/ml) 1 x 10µl 1 x 20µl EAH1 (10X Lysis Buffer) 1 x 5ml 1 x 10ml EAH10 (Stop Solution) 1 x 3ml 1 x 6ml EAH2 (Extraction Buffer) 1 x 8ml 1 x 16ml EAH3 (10X Wash Buffer) 1 x 14ml 1 x 28ml EAH4 (Histone Buffer) 1 x 0.5ml 1 x 1ml EAH5 (Blocking Buffer) 1 x 10ml 1 x 20ml EAH6 (Antibody Buffer) 1 x 6ml 1 x 12ml EAH7 (Capture Antibody, 100 µg/ml) 1 x 25µl 1 x 50µl EAH8 (Detection Antibody, 400 µg/ml) 1 x 10µl 1 x 20µl EAH9 (Developing Solution) 1 x 5ml 1 x 10ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 Acetylation Assay Kit (ab115102)
ab115102 has not yet been referenced specifically in any publications.
