EpiSeeker Histone H3 Acetylation Assay Kit (ab115102)

Overview

  • Product nameEpiSeeker Histone H3 Acetylation Assay KitSee all Histone H3 acetylation kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.

    EpiSeeker Histone H3 Acetylation Assay Kit (ab115102) allows the user to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Notes

    Store the Acetylated Histone H3 Control and EAH8 at –20°C away from light.

    Store EAH3, EAH5, EAH6, EAH7, EAH9, and the 8-Well Assay Strips at 4°C away from light.

    Store all other components at room temperature.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115102 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker Histone H3 Acetylation Assay Kit (ab115102)

    ab115102 has not yet been referenced specifically in any publications.

    Product Wall

    Compared to ab115102, ab115124 is much faster and easier to handle with higher sensitivity and better reproducibility, but slightly less specificity. 1-2 ug of histone protein (min 50,000 cells) may be needed for each assay point if using ab115102, whi...

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    Hello! The two kits ab115102 and ab113476 should be suitable for use in fish, while ab133099 is designed to study inhibitors and so fish is not suitable. Please let me know if you have further questions.

    Thank you for your enquiry and your interest in our products.

    I have discussed your question with some colleagues in the Lab and can confirm that RIPA buffer would not be compatible with the 10X Lysis Buffer.

    If needed, you can purc...

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    Thank you for your inquiry.

    The kits are very similar. The main differences are that the ab115102 requires 5 hrs to run and you need to coat the antibody yourself while the kit ab115124 only requires 2.5 hours and the wells come pre-coated. <...

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    Thank you for contacting Abcam.


    Regarding the use of a 60 or 100mm plate - this is used for disecting tissue samples if you are using them, however, once you proceed with the histone extraction, the assay is then performed in the strip we...

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    Vielen Dank für Ihre Anfrage.

    ab115124 ist schneller und einfacher zu benutzen und besitzt außerdem eine bessere Reproduzierbarkeit. Allerdings ist die Spezifität etwas geringer als beiab115102.

    Fuer ab115102 werden ca. 1-2ug Histon...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"