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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. In mammalian cells, the EZ family group of proteins among others are responsible for the addition of one, two or three methyl groups at lysine 17 of histone H3.
EpiSeeker Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3(K27)). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.
Store EHM3, EHM4, EHM5, EHM7, and the Control Enzyme at –20°C away from light.
Store all other components at 4°C away from light.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Control Enzyme (150 µg/ml)||1 x 10µl||1 x 20µl|
|EHM1 (10X Wash Buffer)||1 x 11ml||1 x 22ml|
|EHM2 (Histone Assay Buffer)||1 x 1.5ml||1 x 3ml|
|EHM3 (Adomet)||1 x 25µl||1 x 50µl|
|EHM4 (Biotinylated Substrate, 25 µg/ml)||1 x 100µl||1 x 200µl|
|EHM5 (HMT Standard, 10 µg/ml)||1 x 10µl||1 x 20µl|
|EHM6 (Capture Antibody, 100 µg/ml)||1 x 25µl||1 x 50µl|
|EHM7 (Detection Antibody, 100 µg/ml)||1 x 10µl||1 x 20µl|
|EHM8 (Developing Solution)||1 x 6ml||1 x 12ml|
|EHM9 (Stop Solution)||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113454 has not yet been referenced specifically in any publications.
Publishing research using ab113454 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"