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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. H3(K4) demethylation is regulated by KDM1/LSD1, which can remove di- and mono-methylation from H3(K4), and the JARID proteins such as RBP2 which catalyzes the removal of methylation through a hydroxylation reaction.
EpiSeeker Histone H3 (K4) Demethylase Activity Quantification Assay Kit (ab113455) allows the user to measure activity/inhibition of H3(K4) specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3(K4) specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
Store EHD3, EHD4, EHD6, and EHD7 at –20°C away from light.
Store all other components at 4°C away from light.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|EHD1 (10X Wash Buffer)||1 x 15ml||1 x 30ml|
|EHD2 (HDM Assay Buffer)||1 x 1.5ml||1 x 3ml|
|EHD3 (HDM Substrate)||1 x 50µl||1 x 100µl|
|EHD4 (HDM Standard, 20 µg/ml)||1 x 25µl||1 x 50µl|
|EHD5 (Capture Antibody, 1000 µg/ml)||1 x 5µl||1 x 10µl|
|EHD6 (Detection Antibody, 200 µg/ml)||1 x 8µl||1 x 16µl|
|EHD7 (Fluoro Developer)||1 x 12µl||1 x 24µl|
|EHD8 (Fluoro Enhancer)||1 x 12µl||1 x 24µl|
|EHD9 (Fluoro Dilution)||1 x 4ml||1 x 8ml|
Our Abpromise guarantee covers the use of ab113455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113455 has not yet been referenced specifically in any publications.
Publishing research using ab113455 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"