EpiSeeker Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)

Overview

  • Product nameEpiSeeker Histone H3 (K4) Methyltransferase Activity Quantification Assay KitSee all Histone Methyltransferase kits ...
  • Assay typeQuantitative
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. In mammalian cells, several SET-domain containing histone lysine methyltransferases such as MLL1 are responsible for adding one, two or three methyl groups at lysine 4 of histone H3.

    EpiSeeker Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452) allows the user to measure the activity or inhibition of individual histone methyltransferases that specifically target histone H3 at lysine 4 (H3(K4)). The kit is ready-to-use and provides all the essential components needed to carry out a successful histone methyltransferase activity/inhibition quantification assay without the need for radioactivity or any special equipment.

  • Notes

    Store EHM3, EHM4, EHM5, EHM7, and the Control Enzyme at

    –20°C away from light.

    Store all other components at 4°C away from light.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studiesmore details

Properties

  • Alternative names
    • HMT

Applications

Our Abpromise guarantee covers the use of ab113452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

EpiSeeker Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit images

  • Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K4 specific histone methyltrasferase activity (SET7/9) was measured.

Protocols

References for EpiSeeker Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452)

ab113452 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry regarding ab113452.
1. The biotinylated substrate is unmethylated histone peptide and used for H3-K9 specific histone methyltransferase reaction.
2. For generating a standard curve, first, dilute HK5 HMT Standard wi...

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Thank you contacting us with these further questions. I have spoken with the lab about a usefull concentration for the purified HMT enzyme. Based on tests, we conclude that the useful concentration range can be from 10 ng to 200 ng/well (30 ul). ...

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Thank you for contacting us. I have had a chance to speak with the lab regarding your question. They have told me that the purified HMT enzyme (H3-K4 specific) can be used in this assay kit. I hope this information is helpful to you. Please do ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"