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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 4 (K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. In mammalian cells, several SET-domain containing histone lysine methyltransferases such as MLL1 are responsible for adding one, two or three methyl groups at lysine 4 of histone H3.
EpiSeeker Histone H3 (K4) Methyltransferase Activity Quantification Assay Kit (ab113452) allows the user to measure the activity or inhibition of individual histone methyltransferases that specifically target histone H3 at lysine 4 (H3(K4)). The kit is ready-to-use and provides all the essential components needed to carry out a successful histone methyltransferase activity/inhibition quantification assay without the need for radioactivity or any special equipment.
Store EHM3, EHM4, EHM5, EHM7, and the Control Enzyme at
–20°C away from light.
Store all other components at 4°C away from light.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Control Enzyme (100 µg/ml)||1 x 10µl||1 x 20µl|
|EHM1 (10X Wash Buffer)||1 x 11ml||1 x 22ml|
|EHM2 (Histone Assay Buffer)||1 x 1.5ml||1 x 3ml|
|EHM3 (Adomet)||1 x 25µl||1 x 50µl|
|EHM4 (Biotinylated Substrate, 25 µg/ml)||1 x 100µl||1 x 200µl|
|EHM5 (HMT Standard, 10 µg/ml)||1 x 10µl||1 x 20µl|
|EHM6 (Capture Antibody, 100 µg/ml)||1 x 25µl||1 x 50µl|
|EHM7 (Detection Antibody, 200 µg/ml)||1 x 10µl||1 x 20µl|
|EHM8 (Developing Solution)||1 x 6ml||1 x 12ml|
|EHM9 (Stop Solution)||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113452 has not yet been referenced specifically in any publications.
Publishing research using ab113452 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"