EpiSeeker Histone H3 (K9) Demethylase Activity Quantification Assay Kit (ab113458)
Overview
- Product nameEpiSeeker Histone H3 (K9) Demethylase Activity Quantification Assay KitSee all Histone Demethylase kits ...
- Assay time1h 30m
- Species reactivityReacts with: Mouse, Human, Plants
Predicted to work with: all Mammals, Other - Product overview
Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. H3(K9) demethylation is regulated by at least two classes of demethylases, JMJD1 (which can remove mono- and di-methylation from H3(K9)) and JMJD2 (which can remove trimethylation from H3(K9)).
EpiSeeker Histone H3 (K9) Demethylase Activity Quantification Assay Kit (ab113458) allows the user to measure activity/inhibition of H3(K9) specific histone demethylases using cell/tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3(K9) specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
- Notes
Store EHD2 at –20°C away from light.
Store all other components at 4°C away from light.
The kit is stable for up to 6 months from the date of shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units EHD1 (HDM Assay Buffer) 1 x 2ml 1 x 4ml EHD2 (HDM Substrate) 1 x 50µl 1 x 100µl EHD3 (HDM Standard, 10 mM) 1 x 25µl 1 x 50µl EHD4 (Fluoro-Developer) 1 x 4ml 1 x 8ml EHD5 (Fluoro-Enhancer) 1 x 4ml 1 x 8ml HDM Cofactor 1 1 x 25µl 1 x 50µl HDM Cofactor 2 1 x 25µl 1 x 50µl HDM Cofactor 3 1 x 25µl 1 x 50µl - Research Areas
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Alternative names
- HDM
Applications
Our Abpromise guarantee covers the use of ab113458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (K9) Demethylase Activity Quantification Assay Kit (ab113458)
ab113458 has not yet been referenced specifically in any publications.
