EpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)
- Product nameEpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay KitSee all Histone Methyltransferase kits ...
- Assay typeQuantitative
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.
EpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3(K9)). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.
ab113453 recognizes methylated H3 at lysine site 9.
Store EHM3, EHM4, EHM5, EHM7, and the Control Enzyme at –20°C away from light.
Store all other components at 4°C away from light.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units Control Enzyme (150 µg/ml) 1 x 13µl 1 x 26µl EHM1 (10X Wash Buffer) 1 x 11ml 1 x 22ml EHM2 (Histone Assay Buffer) 1 x 1.5ml 1 x 3ml EHM3 (Adomet) 1 x 25µl 1 x 50µl EHM4 (Biotinylated Substrate, 25 µg/ml) 1 x 100µl 1 x 200µl EHM5 (HMT Standard, 10 µg/ml) 1 x 10µl 1 x 20µl EHM6 (Capture Antibody, 100 µg/ml) 1 x 25µl 1 x 50µl EHM7 (Detection Antibody, 200 µg/ml) 1 x 10µl 1 x 20µl EHM8 (Developing Solution) 1 x 6ml 1 x 12ml EHM9 (Stop Solution) 1 x 3ml 1 x 6ml
- Research Areas
Our Abpromise guarantee covers the use of ab113453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit images
Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K9 specific histone methyltrasferase activity (G9a) was measured. Purified G9a was spiked into the nuclear extract that has low concentration of nuclear proteins by dilution.
References for EpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)
ab113453 has not yet been referenced specifically in any publications.