EpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)

Overview

  • Product nameEpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay KitSee all Histone Methyltransferase kits ...
  • Assay typeQuantitative
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.

    EpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3(K9)). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.

     

    ab113453 recognizes methylated H3 at lysine site 9.

  • Notes

    Store EHM3, EHM4, EHM5, EHM7, and the Control Enzyme at –20°C away from light.

    Store all other components at 4°C away from light.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studiesmore details

Properties

  • Alternative names
    • HTM

Applications

Our Abpromise guarantee covers the use of ab113453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

EpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit images

  • Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K9 specific histone methyltrasferase activity (G9a) was measured. Purified G9a was spiked into the nuclear extract that has low concentration of nuclear proteins by dilution.

Protocols

References for EpiSeeker Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)

ab113453 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
Here are the answers to your questions:
1. Peptide.
2. Yes, avidin-coated.
3. This information cannot be disclosed currently.
4. No. unless a purified enzyme or extract with the over-expressed speci...

Read More

Thank you for contacting us.
Here are the answers to your questions:
1. After dilution, each diluted component should be used within the same day.
2. At 4C for same day.
3. for longer time storage, as it will not be frozen at -20C. ...

Read More

Thank you for contacting us.
Here are the answers to your questions:
1. 100,000-500,000 cells or 10-50 mg tissues.
2. It is possible but results are not guaranteed.
3. It can be stable for 30 min after adding stop solution.
4. ...

Read More

Thank you for contacting us and thank you for your patience.

I have heard back from the lab with the following information:

1. Yes, this kit is suitable for the conditions mentioned (rat oligodendrocytes with and without inhibitors...

Read More

Thanks for your inquiry. Yes, the kit ab113453 is suitable for measuring H3K9 activity from various species including Drosophila based on the working principle and antibody's specificity. Please let me know if you require further ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"