EpiSeeker Histone H3 (acetyl K18) Quantification Kit (Fluorometric) (ab115109)
Overview
- Product nameEpiSeeker Histone H3 (acetyl K18) Quantification Kit (Fluorometric)See all Histone H3 (K18 acetylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity0.4 ng/well
- Range5 ng/well - 2000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Lysine 18 at histone H3, along with K9 and K14, are primary acetylated sites of histone H3. Histone H3 (acetyl K18) is tightly involved in cell cycle regulation, cell proliferation and apoptosis. It also correlates with transcription activation.
EpiSeeker Histone H3 (acetyl K18) Quantification Kit (Fluorometric) (ab115109) is a convenient package of tools that allows the experimenter to measure global acetylation of histone H3-K18 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H3-K18 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
- Notes
Store ESF3, ESF4, and Standard Control at –20°C away from light.
Store all other components at 4°C away from light.
The components of the kit should be stable for 6 months when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Sample Strips (with frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESF1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESF2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESF3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (Fluoro-Developer) 1 x 12µl 1 x 24µl ESF5 (Fluoro-Enhancer) 1 x 12µl 1 x 24µl ESF6 (Fluoro-Dilution) 1 x 4ml 1 x 8ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (acetyl K18) Quantification Kit (Fluorometric) (ab115109)
ab115109 has not yet been referenced specifically in any publications.
