EpiSeeker Histone H3 (acetyl K36) Quantification Kit (Colorimetric) (ab115112)

Overview

  • Product nameEpiSeeker Histone H3 (acetyl K36) Quantification Kit (Colorimetric)See all Histone H3 (K36 acetylation) kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    2 ng/well
  • Range
    20 ng/well - 5000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone H3 (acetyl K36) is a conserved modification, which functions in transcription. It is also tightly involved in cell cycle regulation, cell proliferation and apoptosis.

    EpiSeeker Histone H3 (acetyl K36) Quantification Kit (Colorimetric) (ab115112) allows the experimenter to measure global acetylation of histone H3-K36 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H3-K36 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Notes

    Upon receipt, store ESC3 and standard control at –20°C. Store all other components at 4°C away from light. The components of the kit are stable for up to 6 months from the date of shipment, when stored properly.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115112 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker Histone H3 (acetyl K36) Quantification Kit (Colorimetric) (ab115112)

    ab115112 has not yet been referenced specifically in any publications.

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