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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone H3 (acetyl K36) is a conserved modification, which functions in transcription. It is also tightly involved in cell cycle regulation, cell proliferation and apoptosis.
EpiSeeker Histone H3 (acetyl K36) Quantification Kit (Colorimetric) (ab115112) allows the experimenter to measure global acetylation of histone H3-K36 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H3-K36 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
Upon receipt, store ESC3 and standard control at –20°C. Store all other components at 4°C away from light. The components of the kit are stable for up to 6 months from the date of shipment, when stored properly.
|Components||48 tests||96 tests|
|8 Well Sample Strips (with Frame)||4 units||9 units|
|8 Well Standard Control Strips||2 units||3 units|
|ESC1 (10X Wash Buffer)||1 x 10ml||1 x 20ml|
|ESC2 (Antibody Buffer)||1 x 6ml||1 x 12ml|
|ESC3 (Detection Antibody, 1 mg/ml)||1 x 5µl||1 x 10µl|
|ESC4 (Color Developer)||1 x 5ml||1 x 10ml|
|ESC5 (Stop Solution)||1 x 3ml||1 x 6ml|
|Standard Control (100 µg/ml)*||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115112 has not yet been referenced specifically in any publications.
Publishing research using ab115112 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"