EpiSeeker Histone H3 (acetyl K56) Quantification Kit (Fluorometric) (ab115115)
Overview
- Product nameEpiSeeker Histone H3 (acetyl K56) Quantification Kit (Fluorometric)See all Histone H3 (K56 acetylation) kits ...
- Sample typeAdherent cells, Suspension cells
- Sensitivity0.4 ng/well
- Range5 ng/well - 2000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone H3 (acetyl K56) is cell cycle regulated, maintained upon replicative damage in a check-point manner and is essential for surviving DNA damage. H3 (acetyl K56) occurs normally during S-phase, and in absence of this modification, cellular survival following DNA damage is impaired.
EpiSeeker Histone H3 (acetyl K56) Quantification Kit (Fluorometric) (ab115115) allows the experimenter to measure global acetylation of histone H3-K56 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H3-K56 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
- Notes
Upon receipt, store ESF3, ESF4 and Standard control at –20°.
Store all other components at 4°C away from light. The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESF1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESF2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESF3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (Fluoro Developer) 1 x 12µl 1 x 24µl ESF5 (Fluoro Enhancer) 1 x 12µl 1 x 24µl ESF6 (Fluoro Dilution) 1 x 4ml 1 x 8ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (acetyl K56) Quantification Kit (Fluorometric) (ab115115)
ab115115 has not yet been referenced specifically in any publications.
