EpiSeeker Histone H3 (acetyl K9) Assay Kit (In Situ) (ab115101)
- Product nameEpiSeeker Histone H3 (acetyl K9) Assay Kit (In Situ)See all Histone H3 (K9 acetylation) kits ...
- Sample typeAdherent cells
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
Acetylation of histone H3 at lysine 9 (K9) is a mark of active chromatin and appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Increased H3 (acetyl K9) may also reflect a decrease in H3 (methyl K9) associated with gene repression.
EpiSeeker Histone H3 (acetyl K9) Assay Kit (In Situ) (ab115101) allows the user to measure in situ acetylation of histone H3-K9 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115101 is suitable for specifically measuring in situ histone H3-K9 acetylation using cultured adherent cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 96 tests 2 x 96 tests 30% H2O2 Solution 1 x 0.5ml 1 x 1ml 8-Well Control Strips 2 units 4 units Acetylated H3-K9 Control (20 µg/ml) 1 x 15µl 1 x 30µl EAA1 (10X Wash Buffer) 1 x 30ml 2 x 30ml EAA2 (Permeabilizing Buffer) 1 x 30ml 2 x 30ml EAA3 (Blocking Buffer) 1 x 20ml 2 x 20ml EAA4 (Antibody Buffer) 1 x 15ml 1 x 30ml EAA5 (Capture Antibody, 1 mg/ml) 1 x 6µl 1 x 10µl EAA6 (Detection Antibody, 400 µg/ml) 1 x 20µl 1 x 40µl EAA7 (Developing Solution) 1 x 12ml 1 x 24ml EAA8 (Stop Solution) 1 x 6ml 1 x 12ml Microplates 1 unit 2 units
- Research Areas
Our Abpromise guarantee covers the use of ab115101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for EpiSeeker Histone H3 (acetyl K9) Assay Kit (In Situ) (ab115101)
ab115101 has not yet been referenced specifically in any publications.