EpiSeeker Histone H3 (acetyl K9) Assay Kit (In Situ) (ab115101)

Overview

• Product nameEpiSeeker Histone H3 (acetyl K9) Assay Kit (In Situ)See all Histone H3 (K9 acetylation) kits ...
• Sample type
  Adherent cells
• Assay time
  3h 00m
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: all Mammals
• Product overview

  Acetylation of histone H3 at lysine 9 (K9) is a mark of active chromatin and appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Increased H3 (acetyl K9) may also reflect a decrease in H3 (methyl K9) associated with gene repression.

  EpiSeeker Histone H3 (acetyl K9) Assay Kit (In Situ) (ab115101) allows the user to measure in situ acetylation of histone H3-K9 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115101 is suitable for specifically measuring in situ histone H3-K9 acetylation using cultured adherent cells.

• Tested applicationsFunctional Studies more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	96 tests	2 x 96 tests
  30% H2O2 Solution	1 x 0.5ml	1 x 1ml
  8-Well Control Strips	2 units	4 units
  Acetylated H3-K9 Control (20 µg/ml)	1 x 15µl	1 x 30µl
  EAA1 (10X Wash Buffer)	1 x 30ml	2 x 30ml
  EAA2 (Permeabilizing Buffer)	1 x 30ml	2 x 30ml
  EAA3 (Blocking Buffer)	1 x 20ml	2 x 20ml
  EAA4 (Antibody Buffer)	1 x 15ml	1 x 30ml
  EAA5 (Capture Antibody, 1 mg/ml)	1 x 6µl	1 x 10µl
  EAA6 (Detection Antibody, 400 µg/ml)	1 x 20µl	1 x 40µl
  EAA7 (Developing Solution)	1 x 12ml	1 x 24ml
  EAA8 (Stop Solution)	1 x 6ml	1 x 12ml
  Microplates	1 unit	2 units

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • Epigenetic kits
  • Histone acetylation and deacetylation

  • Epigenetics and Nuclear Signaling
  • Histones
  • H3
  • Acetylated

Applications

Protocols

Protocol Booklet