EpiSeeker Histone H3 (acetyl K9) Quantification Kit (Colorimetric) (ab115104)
Overview
- Product nameEpiSeeker Histone H3 (acetyl K9) Quantification Kit (Colorimetric)See all Histone H3 (K9 acetylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity> 2 ng/well
- Range20 ng/well - 5000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 acetylation. Lysine 9 at histone H3 is a primary acetylated site of histone H3. Histone H3 (acetyl K9) appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Histone H3 (acetyl K9) is an active marker as well as being tightly involved in cell cycle regulation, cell proliferation and apoptosis. The balance between H3 (acetyl K9) and H3 (methyl K9) is important for the establishment of specific chromatin structures.
EpiSeeker Histone H3 (acetyl K9) Quantification Kit (Colorimetric) (ab115104) allows the user to measure global acetylation of histone H3-K9. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3-K9 acetylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Notes
Store ESC3 and Standard Control at –20°C.
Store all other components at 4°C away from light.
The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESC1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESC2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESC3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESC4 (Color Developer) 1 x 5ml 1 x 10µl ESC5 (Stop Solution) 1 x 3ml 1 x 6ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (acetyl K9) Quantification Kit (Colorimetric) (ab115104)
ab115104 has not yet been referenced specifically in any publications.
