EpiSeeker Histone H3 (di-methyl K27) Quantification Kit (Fluorometric) (ab115071)
Overview
- Product nameEpiSeeker Histone H3 (di-methyl K27) Quantification Kit (Fluorometric)See all Histone H3 (K27 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (di-methyl K27), a modification enriched at pericentromic heterochromatin, is observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.
EpiSeeker Histone H3 (di-methyl K27) Quantification Kit (Fluorometric) (ab115071) allows the user to specifically measure global di-methylation of histone H3-K27 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8 well sample strips (with frame) 4 units 9 units 8 well standard control strips 2 units 3 units ESF1 (10X wash buffer) 1 x 10ml 1 x 20ml ESF2 (antibody buffer) 1 x 6ml 1 x 12ml ESF3 (detection antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (fluoro developer) 1 x 12µl 1 x 24µl ESF5 (fluoro enhancer) 1 x 12µl 1 x 24µl ESF6 (fluoro dilution) 1 x 4ml 1 x 8ml Standard control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (di-methyl K27) Quantification Kit (Fluorometric) (ab115071)
ab115071 has not yet been referenced specifically in any publications.
