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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. H3 (di-methyl K4) is associated with transcriptionally permissive euchromatin regions in the genome; in particular, Enrichment of H3 (di-methyl K4) is present across the body of genes, where contributes to mRNA processing.
EpiSeeker Histone H3 (di-methyl K4) Quantification Kit (Fluorometric) (ab115053) allows the user to specifically measure global di-methylation of histone H3-K4 fluorometrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|8-Well Sample Strips (with frame)||4 units||9 units|
|8-Well Standard Control Strips||2 units||3 units|
|ESF1 (10X Wash Buffer)||1 x 10ml||1 x 20ml|
|ESF2 (Antibody Buffer)||1 x 6ml||1 x 12ml|
|ESF3 (Detection Antibody, 1 mg/ml)||1 x 5µl||1 x 10µl|
|ESF4 (Fluoro Developer)||1 x 12µl||1 x 24µl|
|ESF5 (Fluoro Enhancer)||1 x 12µl||1 x 24µl|
|ESF6 (Fluoro Dilution)||1 x 4ml||1 x 8ml|
|Standard Control (100 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115053 has not yet been referenced specifically in any publications.
Publishing research using ab115053 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"