EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)
Overview
- Product nameEpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric)See all Histone H3 (K9 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as in heterochromatin formation. Specifically, H3 (di-methyl K9) mediates heterochromatin formation by forming a binding site for HP1.
EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062) allows the user to specifically measure global di-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Sample Strips (with frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESC1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESC2 (Antibody Buffer) 1 x 6ml 1 x 2ml ESC3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESC4 (Color Developer) 1 x 5ml 1 x 10ml ESC5 (Stop Solution) 1 x 3ml 1 x 6ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) images
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Functional Studies - EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)Histone extracts were prepared from MDA-MB-231 cells using the EpiSeeker Histone Extraction Kit (ab113476) and the amount of dimethyl-H3-K9 was measured using ab115062.
Protocols
References for EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)
ab115062 has not yet been referenced specifically in any publications.
