EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)

Overview

  • Product nameEpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric)See all Histone H3 (K9 methylation) kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as in heterochromatin formation. Specifically, H3 (di-methyl K9) mediates heterochromatin formation by forming a binding site for HP1.

    EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062) allows the user to specifically measure global di-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115062 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) images

    • Histone extracts were prepared from MDA-MB-231 cells using the EpiSeeker Histone Extraction Kit (ab113476) and the amount of dimethyl-H3-K9 was measured using ab115062.

    Protocols

    References for EpiSeeker Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)

    ab115062 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"