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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting.
EpiSeeker Histone H3 (methyl K27) Assay Kit (ab115050) allows the user to specifically measure global histone H3-K27 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|EGG1 (10X Lysis Buffer)||1 x 5ml||1 x 10ml|
|EGG10 (Stop Solution)||1 x 3ml||1 x 6ml|
|EGG2 (Extraction Buffer)||1 x 8ml||1 x 16ml|
|EGG3 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|EGG4 (Histone Buffer)||1 x 0.5ml||1 x 1ml|
|EGG5 (Blocking Buffer)||1 x 10ml||1 x 20ml|
|EGG6 (Antibody Buffer)||1 x 6ml||1 x 12ml|
|EGG7 (Capture Antibody, 100 µg/ml)||1 x 25µl||1 x 50µl|
|EGG8 (Detection Antibody, 400 µg/ml)||1 x 10µl||1 x 20µl|
|EGG9 (Developing Solution)||1 x 5ml||1 x 10ml|
|Methylated H3-K27 Control (60 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115050 has not yet been referenced specifically in any publications.
Publishing research using ab115050 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"