EpiSeeker Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046)

Overview

• Product nameEpiSeeker Histone H3 (methyl K4) Assay Kit (In Situ)See all Histone H3 (K4 methylation) kits ...
• Sample type
  Adherent cells
• Assay time
  3h 00m
• Product overview

  Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions and may serve as a global epigenetic mark in euchromatin. H3 (methyl K4) also appears to have an inverse correlation to allele-specific DNA methylation.

   

  EpiSeeker Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046) allows the user to specifically measure histone H3-K4 methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

• Notes

  Store EGA6 and Methylated H3-K4 Control at –20°C. Store EGA1, EGA3, EGA5, EGA7 and the 8-well control strips at 4°C away from light. Store all other components at room temperature. The kit is stable for up to 6 months from the shipment date, when stored properly.

• Tested applicationsFunctional Studies more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	96 tests	2 x 96 tests
  30% H2O2 Solution	1 x 0.5ml	1 x 1ml
  8-Well Control Strips	2 units	4 units
  EGA1 (10X Wash Buffer)	1 x 30ml	2 x 30ml
  EGA2 (Permeabilizing Buffer)	1 x 30ml	2 x 30ml
  EGA3 (Blocking Buffer)	1 x 20ml	2 x 20ml
  EGA4 (Antibody Buffer)	1 x 10ml	1 x 20ml
  EGA5 (Capture Antibody, 100 µg/ml)	1 x 60µl	1 x 120µl
  EGA6 (Detection Antibody, 200 µg/ml)	1 x 20µl	1 x 40µl
  EGA7 (Developing Solution)	1 x 12ml	1 x 24ml
  EGA8 (Stop Solution)	1 x 6ml	1 x 12ml
  Methylated H3-K4 Control (20 µg/ml)	1 x 15µl	1 x 30µl
  Microplates	1 unit	2 units

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • Epigenetic kits
  • Histone methylation quantification

  • Epigenetics and Nuclear Signaling
  • Histones
  • H3
  • Methylated

Applications

Protocols

Protocol Booklet