EpiSeeker Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046)
- Product nameEpiSeeker Histone H3 (methyl K4) Assay Kit (In Situ)See all Histone H3 (K4 methylation) kits ...
- Sample typeAdherent cells
- Assay time3h 00m
- Product overview
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions and may serve as a global epigenetic mark in euchromatin. H3 (methyl K4) also appears to have an inverse correlation to allele-specific DNA methylation.
EpiSeeker Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046) allows the user to specifically measure histone H3-K4 methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.
Store EGA6 and Methylated H3-K4 Control at –20°C. Store EGA1, EGA3, EGA5, EGA7 and the 8-well control strips at 4°C away from light. Store all other components at room temperature. The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 96 tests 2 x 96 tests 30% H2O2 Solution 1 x 0.5ml 1 x 1ml 8-Well Control Strips 2 units 4 units EGA1 (10X Wash Buffer) 1 x 30ml 2 x 30ml EGA2 (Permeabilizing Buffer) 1 x 30ml 2 x 30ml EGA3 (Blocking Buffer) 1 x 20ml 2 x 20ml EGA4 (Antibody Buffer) 1 x 10ml 1 x 20ml EGA5 (Capture Antibody, 100 µg/ml) 1 x 60µl 1 x 120µl EGA6 (Detection Antibody, 200 µg/ml) 1 x 20µl 1 x 40µl EGA7 (Developing Solution) 1 x 12ml 1 x 24ml EGA8 (Stop Solution) 1 x 6ml 1 x 12ml Methylated H3-K4 Control (20 µg/ml) 1 x 15µl 1 x 30µl Microplates 1 unit 2 units
- Research Areas
Our Abpromise guarantee covers the use of ab115046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for EpiSeeker Histone H3 (methyl K4) Assay Kit (In Situ) (ab115046)
ab115046 has not yet been referenced specifically in any publications.