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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions and may serve as a global epigenetic mark in euchromatin. H3 (methyl K4) also appears to have an inverse correlation to allele-specific DNA methylation.
EpiSeeker Histone H3 (methyl K4) Assay Kit (ab115048) allows the user to specifically measure global histone H3-K4 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|EGD1 (10X Lysis Buffer)||1 x 5ml||1 x 10ml|
|EGD10 (Stop Solution)||1 x 3ml||1 x 6ml|
|EGD2 (Extraction Buffer)||1 x 8ml||1 x 16ml|
|EGD3 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|EGD4 (Histone Buffer)||1 x 0.5ml||1 x 1ml|
|EGD5 (Blocking Buffer)||1 x 10ml||1 x 20ml|
|EGD6 (Antibody Buffer)||1 x 6ml||1 x 12ml|
|EGD7 (Capture Antibody, 100 µg/ml)||1 x 25µl||1 x 50µl|
|EGD8 (Detection Antibody, 400 µg/ml)||1 x 10µl||1 x 20µl|
|EGD9 (Developing Solution)||1 x 5ml||1 x 10ml|
|Methylated H3-K4 Control (60 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115048 has not yet been referenced specifically in any publications.
Publishing research using ab115048 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"