EpiSeeker Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047)

Overview

  • Product nameEpiSeeker Histone H3 (methyl K9) Assay Kit (In Situ)See all Histone H3 (K9 methylation) kits ...
  • Sample type
    Adherent cells
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. Moreover, presence of H3 (methyl K9) positively correlates with DNA methylation.

    EpiSeeker Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047) allows the user to specifically measure histone H3-K9 methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsPlease refer to protocols.
  • Components 96 tests 2 x 96 tests
    30% H2O2 Solution 1 x 0.5ml 1 x 1ml
    8-Well Control Strips 2 units 4 units
    EGB1 (10X Wash Buffer) 1 x 30ml 2 x 30ml
    EGB2 (Permeabilizing Buffer) 1 x 30ml 2 x 30ml
    EGB3 (Blocking Buffer) 1 x 20ml 2 x 20ml
    EGB4 (Antibody Buffer) 1 x 15ml 1 x 20ml
    EGB5 (Capture Antibody, 100 µg/ml) 1 x 60µl 1 x 120µl
    EGB6 (Detection Antibody, 400 µg/ml) 10 x 20µl 1 x 40µl
    EGB7 (Developing Solution) 1 x 12ml 1 x 24ml
    EGB8 (Stop Solution) 1 x 6ml 1 x 12ml
    Methylated H3-K9 Control (20 µg/ml) 1 x 15µl 1 x 30µl
    Microplates 1 unit 2 units
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab115047 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047)

    ab115047 has not yet been referenced specifically in any publications.

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