EpiSeeker Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047)
Overview
- Product nameEpiSeeker Histone H3 (methyl K9) Assay Kit (In Situ)See all Histone H3 (K9 methylation) kits ...
- Sample typeAdherent cells
- Assay time3h 00m
- Species reactivityReacts with: Mouse
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. Moreover, presence of H3 (methyl K9) positively correlates with DNA methylation.
EpiSeeker Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047) allows the user to specifically measure histone H3-K9 methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 96 tests 2 x 96 tests 30% H2O2 Solution 1 x 0.5ml 1 x 1ml 8-Well Control Strips 2 units 4 units EGB1 (10X Wash Buffer) 1 x 30ml 2 x 30ml EGB2 (Permeabilizing Buffer) 1 x 30ml 2 x 30ml EGB3 (Blocking Buffer) 1 x 20ml 2 x 20ml EGB4 (Antibody Buffer) 1 x 15ml 1 x 20ml EGB5 (Capture Antibody, 100 µg/ml) 1 x 60µl 1 x 120µl EGB6 (Detection Antibody, 400 µg/ml) 10 x 20µl 1 x 40µl EGB7 (Developing Solution) 1 x 12ml 1 x 24ml EGB8 (Stop Solution) 1 x 6ml 1 x 12ml Methylated H3-K9 Control (20 µg/ml) 1 x 15µl 1 x 30µl Microplates 1 unit 2 units - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (methyl K9) Assay Kit (In Situ) (ab115047)
ab115047 has not yet been referenced specifically in any publications.
