Overview
- Product nameEpiSeeker Histone H3 (methyl K9) Assay KitSee all Histone H3 (K9 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. Moreover, presence of H3 (methyl K9) positively correlates with DNA methylation.
EpiSeeker Histone H3 (methyl K9) Assay Kit (ab115049) allows the user to specifically measure global histone H3-K9 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units EGC1 (10X Lysis Buffer) 1 x 5ml 1 x 10ml EGC10 (Stop Solution) 1 x 3ml 1 x 6ml EGC2 (Extraction Buffer) 1 x 8ml 1 x 16ml EGC3 (10X Wash Buffer) 1 x 14ml 1 x 28ml EGC4 (Histone Buffer) 1 x 0.5ml 1 x 1ml EGC5 (Blocking Buffer) 1 x 10ml 1 x 20ml EGC6 (Antibody Buffer) 1 x 6ml 1 x 12ml EGC7 (Capture Antibody, 100 µg/ml) 1 x 25µl 1 x 50µl EGC8 (Detection Antibody, 400 µg/ml) 1 x 10µl 1 x 20µl EGC9 (Developing Solution) 1 x 5ml 1 x 10ml Methylated H3-K9 Control (60 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (methyl K9) Assay Kit (ab115049)
ab115049 has not yet been referenced specifically in any publications.
