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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. Moreover, presence of H3 (methyl K9) positively correlates with DNA methylation.
EpiSeeker Histone H3 (methyl K9) Assay Kit (ab115049) allows the user to specifically measure global histone H3-K9 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|EGC1 (10X Lysis Buffer)||1 x 5ml||1 x 10ml|
|EGC10 (Stop Solution)||1 x 3ml||1 x 6ml|
|EGC2 (Extraction Buffer)||1 x 8ml||1 x 16ml|
|EGC3 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|EGC4 (Histone Buffer)||1 x 0.5ml||1 x 1ml|
|EGC5 (Blocking Buffer)||1 x 10ml||1 x 20ml|
|EGC6 (Antibody Buffer)||1 x 6ml||1 x 12ml|
|EGC7 (Capture Antibody, 100 µg/ml)||1 x 25µl||1 x 50µl|
|EGC8 (Detection Antibody, 400 µg/ml)||1 x 10µl||1 x 20µl|
|EGC9 (Developing Solution)||1 x 5ml||1 x 10ml|
|Methylated H3-K9 Control (60 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115049 has not yet been referenced specifically in any publications.
Publishing research using ab115049 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"