EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069)
Overview
- Product nameEpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric)See all Histone H3 (K27 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity0.5 ng/well
- Range20 ng/well - 5000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. H3 (methyl K27) is also thought to have an important role in the repression of HOX genes during development and in X chromosome inactivation and imprinting. H3 (mono-methyl K27),a modification enriched at pericentromeric heterochromatin, was observed to be broadly distributed throughout all euchromatic sites and participates in silencing gene expression.
EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069) allows the user to specifically measure global mono-methylation of histone H3-K27 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Notes
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESF1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESF2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESF3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (Fluoro-Developer) 1 x 12µl 1 x 24µl ESF5 (Fluoro-Enhancer) 1 x 12µl 1 x 24µl ESF6 (Fluoro-Dilution) 1 x 4ml 1 x 8ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) images
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Functional Studies - EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069)ab115069 used to measure the amount of mono-methyl-H3-K27. Histone extracts were prepared from MDA-MB-231 cells using the EpiSeeker Histone Extraction Kit (ab113476).
Protocols
References for EpiSeeker Histone H3 (mono-methyl K27) Quantification Kit (Fluorometric) (ab115069)
ab115069 has not yet been referenced specifically in any publications.
