EpiSeeker Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084)

Overview

• Product nameEpiSeeker Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric)See all Histone H3 (K79 methylation) kits ...
• Sample type
  Tissue, Adherent cells, Suspension cells
• Sensitivity
  0.4 ng/well
• Range
  5 ng/well - 2000 ng/well
• Assay time
  2h 30m
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: all Mammals
• Product overview

  Methylation of histone H3 at lysine 79 is catalyzed by Dot1 in mammalian cells. Intergenic H3 (mono-methyl K79) is a mark of enhancer regions and may provide unique binding surfaces along the chromatin fiber to stabilize interactions between enhancers and their target promoters.

  EpiSeeker Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084) allows the user to specifically measure global mono-methylation of histone H3-K79 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

• Tested applicationsFunctional Studies more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	48 tests	96 tests
  8-Well Sample Strips (with Frame)	4 units	9 units
  8-Well Standard Control Strips	2 units	3 units
  ESF1 (10X Wash Buffer)	1 x 10ml	1 x 20ml
  ESF2 (Antibody Buffer)	1 x 6ml	1 x 12ml
  ESF3 (Detection Antibody, 1 mg/ml)	1 x 5µl	1 x 10µl
  ESF4 (Fluoro Developer)	1 x 12µl	1 x 24µl
  ESF5 (Fluoro Enhancer)	1 x 12µl	1 x 24µl
  ESF6 (Fluoro Dilution)	1 x 4ml	1 x 8ml
  Standard Control (100 µg/ml)	1 x 10µl	1 x 20µl

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • Epigenetic kits
  • Histone methylation quantification

  • Epigenetics and Nuclear Signaling
  • Assays & Kits
  • Histone methylation quantification

  • Epigenetics and Nuclear Signaling
  • Histones
  • H3
  • Methylated

Applications

Protocols

Protocol Booklet