EpiSeeker Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084)
- Product nameEpiSeeker Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric)See all Histone H3 (K79 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity0.4 ng/well
- Range5 ng/well - 2000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
Methylation of histone H3 at lysine 79 is catalyzed by Dot1 in mammalian cells. Intergenic H3 (mono-methyl K79) is a mark of enhancer regions and may provide unique binding surfaces along the chromatin fiber to stabilize interactions between enhancers and their target promoters.
EpiSeeker Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084) allows the user to specifically measure global mono-methylation of histone H3-K79 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESF1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESF2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESF3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (Fluoro Developer) 1 x 12µl 1 x 24µl ESF5 (Fluoro Enhancer) 1 x 12µl 1 x 24µl ESF6 (Fluoro Dilution) 1 x 4ml 1 x 8ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl
- Research Areas
Our Abpromise guarantee covers the use of ab115084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for EpiSeeker Histone H3 (mono-methyl K79) Quantification Kit (Fluorometric) (ab115084)
ab115084 has not yet been referenced specifically in any publications.