EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) (ab115060)
Overview
- Product nameEpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric)See all Histone H3 (K9 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases mediates heterochromatin formation by forming a binding site for HP1. H3 (mono-methyl K9) is enriched in certain euchromatin domains which have been postulated to be transcriptionally silent.
EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) (ab115060) allows the user to specifically measure global mono-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
-
Components 48 tests 96 tests 8 well sample strips (with frame) 4 units 9 units 8 well standard control strips 2 units 3 units ESC1 (10X wash buffer) 1 x 10ml 1 x 20ml ESC2 (antibody buffer) 1 x 6ml 1 x 12ml ESC3 (detection antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESC4 (color developer) 1 x 5ml 1 x 10ml ESC5 (stop solution) 1 x 3ml 1 x 6ml Standard control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) images
-
Functional Studies - EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) (ab115060)Histone extracts were prepared from MDA-MB-231 cells using the EpiSeeker Histone Extraction Kit (ab113476) and the amount of monomethyl-H3-K9 was measured using ab115060.
Protocols
References for EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Colorimetric) (ab115060)
ab115060 has not yet been referenced specifically in any publications.
