EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric) (ab115061)
- Product nameEpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric)See all Histone H3 (K9 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases mediates heterochromatin formation by forming a binding site for HP1. H3 (mono-methyl K9) is enriched in certain euchromatin domains which have been postulated to be transcriptionally silent.
EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric) (ab115061) allows the user to specifically measure global mono-methylation of histone H3-K9 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESF1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESF2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESF3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (Fluoro-Developer) 1 x 12µl 1 x 24µl ESF5 (Fluoro-Enhancer) 1 x 12µl 1 x 24µl ESF6 (Fluoro-Dilution) 1 x 4ml 1 x 8ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl
- Research Areas
Our Abpromise guarantee covers the use of ab115061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric) images
Histone extracts were prepared from MDA-231 cells using the EpiSeeker Histone Extraction Kit (ab113476) and the amount of monomethyl-H3-K9 was measured using ab115061.
References for EpiSeeker Histone H3 (mono-methyl K9) Quantification Kit (Fluorometric) (ab115061)
ab115061 has not yet been referenced specifically in any publications.