EpiSeeker Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric) (ab115074)

Overview

• Product nameEpiSeeker Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric)See all Histone H3 (K27 methylation) kits ...
• Tests
  1 x 96 test
• Sample type
  Tissue, Adherent cells, Suspension cells
• Assay time
  2h 30m
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: all Mammals
• Product overview

  Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. H3 (mono-methyl K27), a modification enriched at periocentromic heterochromatin, is broadly distributed throughout all euchromatic sites and participates in silencing gene expression. H3 (di-methyl K27) and H3 (tri-methyl K27) are the facultative heterochromatin mark which promotes the recruitment of PcG proteins for gene silencing.

  EpiSeeker Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric) (ab115074) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

• Tested applicationsFunctional Studies more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	96 tests
  8 well sample strips (with frame)	1 x 9 units
  8 well standard control strips	1 x 3 units
  ESC1 (10X wash buffer)	1 x 20ml
  ESC2 (antibody buffer)	1 x 12ml
  ESC3 (detection antibody, 1 mg/ml)	1 x 10µl
  ESC4 (color developer)	1 x 10ml
  ESC5 (stop solution)	1 x 6ml
  Standard control (100 µg/ml)	1 x 20µl

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • Epigenetic kits
  • Histone methylation quantification

  • Epigenetics and Nuclear Signaling
  • Assays & Kits
  • Histone methylation quantification

  • Epigenetics and Nuclear Signaling
  • Histones
  • H3
  • Methylated

Applications

Protocols

Protocol Booklet