EpiSeeker Histone H3 (pan-methyl K27) Quantification Kit (Fluorometric) (ab131560)
Overview
- Product nameEpiSeeker Histone H3 (pan-methyl K27) Quantification Kit (Fluorometric)See all Histone H3 (K27 methylation) kits ...
- Tests1 x 96 test
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity> 0.4 ng/well
- Range5 ng/well - 2000 ng/well
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. H3 (mono-methyl K27), a modification enriched at periocentromic heterochromatin, is broadly distributed throughout all euchromatic sites and participates in silencing gene expression. H3 (di-methyl K27) and H3 (tri-methyl K27) are the facultative heterochromatin mark which promotes the recruitment of PcG proteins for gene silencing.
ab131560 allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K27 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 96 tests 8-Well Assay Strips (with Frame) 12 units 8-Well Standard Control Strips 3 units ESF1 (10X Wash Buffer) 1 x 20ml ESF2 (Antibody Buffer) 1 x 12ml ESF3 (Detection Antibody, 1 mg/ml) 1 x 10µl ESF4 (Fluoro Developer) 1 x 24µl ESF5 (Fluoro Enhancer) 1 x 24µl ESF6 (Fluoro Dilution) 1 x 8ml Standard Control (100 µg/ml) 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab131560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (pan-methyl K27) Quantification Kit (Fluorometric) (ab131560)
ab131560 has not yet been referenced specifically in any publications.
