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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes. However, when present within protein-coding regions it prevents inappropriate transcriptional initiation of intragenic sequences. H3 (mono-methyl K36) is commonly found together with H3 (di-methyl K27) and associated with transcriptionally silenced genes. However, H3 (di-methyl K36) and H3 (tri-methyl K36) are generally associated with transcriptionally active genes.
EpiSeeker Histone H3 (pan-methyl K36) Quantification Kit (Fluorometric) (ab115082) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K36 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|8 well sample strips (with frame)||1 x 9 units|
|8 well standard control strips||1 x 3 units|
|ESF1 (10X wash buffer)||1 x 20ml|
|ESF2 (antibody buffer)||1 x 12ml|
|ESF3 (detection antibody, 1 mg/ml)||1 x 10µl|
|ESF4 (fluoro developer)||1 x 24µl|
|ESF5 (fluoro enhancer)||1 x 24µl|
|ESF6 (fluoro dilution)||1 x 8ml|
|Standard control (100 µg/ml)||1 x 20µl|
Our Abpromise guarantee covers the use of ab115082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115082 has not yet been referenced specifically in any publications.
Publishing research using ab115082 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"