EpiSeeker Histone H3 (pan-methyl K36) Quantification Kit (Fluorometric) (ab115082)
- Product nameEpiSeeker Histone H3 (pan-methyl K36) Quantification Kit (Fluorometric)See all Histone H3 (K36 methylation) kits ...
- Tests1 x 96 test
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes. However, when present within protein-coding regions it prevents inappropriate transcriptional initiation of intragenic sequences. H3 (mono-methyl K36) is commonly found together with H3 (di-methyl K27) and associated with transcriptionally silenced genes. However, H3 (di-methyl K36) and H3 (tri-methyl K36) are generally associated with transcriptionally active genes.
EpiSeeker Histone H3 (pan-methyl K36) Quantification Kit (Fluorometric) (ab115082) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K36 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 96 tests 8 well sample strips (with frame) 1 x 9 units 8 well standard control strips 1 x 3 units ESF1 (10X wash buffer) 1 x 20ml ESF2 (antibody buffer) 1 x 12ml ESF3 (detection antibody, 1 mg/ml) 1 x 10µl ESF4 (fluoro developer) 1 x 24µl ESF5 (fluoro enhancer) 1 x 24µl ESF6 (fluoro dilution) 1 x 8ml Standard control (100 µg/ml) 1 x 20µl
- Research Areas
Our Abpromise guarantee covers the use of ab115082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for EpiSeeker Histone H3 (pan-methyl K36) Quantification Kit (Fluorometric) (ab115082)
ab115082 has not yet been referenced specifically in any publications.