EpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric) (ab115058)
Overview
- Product nameEpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric)See all Histone H3 (K4 methylation) kits ...
- Tests1 x 96 test
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. In mammals, H3(mono-methyl K4) is associated with silenced euchromatin regions whereas H3(di-methyl K4) and H3(tri-methyl K4) is associated with transcriptionally active euchromatin regions.
EpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric) (ab115058) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 96 tests 8-Well Sample Strips (with Frame) 1 x 9 units 8-Well Standard Control Strips 1 x 3 units ESC1 (10X Wash Buffer) 1 x 20ml ESC2 (Antibody Buffer) 1 x 12ml ESC3 (Detection Antibody, 1 mg/ml) 1 x 10µl ESC4 (Color Developer) 1 x 10ml ESC5 (Stop Solution) 1 x 6ml Standard Control (100 µg/ml) 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric) (ab115058)
ab115058 has not yet been referenced specifically in any publications.
