EpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059)

Overview

• Product nameEpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric)See all Histone H3 (K4 methylation) kits ...
• Tests
  1 x 96 test
• Sample type
  Tissue, Adherent cells, Suspension cells
• Assay time
  2h 30m
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: all Mammals
• Product overview

  Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. In mammals, H3(mono-methyl K4) is associated with silenced euchromatin regions whereas H3(di-methyl K4) and H3(tri-methyl K4) is associated with transcriptionally active euchromatin regions.

  EpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric)(ab115059) allows the user to specifically measure global mono-, di- and tri-methylation of histone H3-K4 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

• Tested applicationsFunctional Studies more details

Properties

• Storage instructionsPlease refer to protocols.

• Components	96 tests
  8 well sample strips (with frame)	1 x 9 units
  8 well standard control strips	1 x 3 units
  ESF1 (10X wash buffer)	1 x 20ml
  ESF2 (antibody buffer)	1 x 12ml
  ESF3 (detection antibody, 1 mg/ml)	1 x 10µl
  ESF4 (fluoro-developer)	1 x 24µl
  ESF5 (fluoro enhancer)	1 x 24µl
  ESF6 (fluoro-dilution)	1 x 8ml
  Standard control (100 µg/ml)	1 x 20µl

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • Epigenetic kits
  • Histone methylation quantification

  • Epigenetics and Nuclear Signaling
  • Assays & Kits
  • Histone methylation quantification

  • Epigenetics and Nuclear Signaling
  • Histones
  • H3
  • Methylated

Applications

Protocols

Protocol Booklet