EpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059)
- Product nameEpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric)See all Histone H3 (K4 methylation) kits ...
- Tests1 x 96 test
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. In mammals, H3(mono-methyl K4) is associated with silenced euchromatin regions whereas H3(di-methyl K4) and H3(tri-methyl K4) is associated with transcriptionally active euchromatin regions.
EpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric)(ab115059) allows the user to specifically measure global mono-, di- and tri-methylation of histone H3-K4 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 96 tests 8 well sample strips (with frame) 1 x 9 units 8 well standard control strips 1 x 3 units ESF1 (10X wash buffer) 1 x 20ml ESF2 (antibody buffer) 1 x 12ml ESF3 (detection antibody, 1 mg/ml) 1 x 10µl ESF4 (fluoro-developer) 1 x 24µl ESF5 (fluoro enhancer) 1 x 24µl ESF6 (fluoro-dilution) 1 x 8ml Standard control (100 µg/ml) 1 x 20µl
- Research Areas
Our Abpromise guarantee covers the use of ab115059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for EpiSeeker Histone H3 (pan-methyl K4) Quantification Kit (Fluorometric) (ab115059)
ab115059 has not yet been referenced specifically in any publications.