EpiSeeker Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) (ab115067)
Overview
- Product nameEpiSeeker Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric)See all Histone H3 (K9 methylation) kits ...
- Tests1 x 96 test
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity0.4 ng/well
- Range5 ng/well - 2000 ng/well
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. H3 (mono-methyl K9) is enriched in certain euchromatic domains, which have been postulated to be transcriptionally silent. H3 (di-methyl K9) and H3 (tri-methyl K9) mediate heterochromatin formation by forming a binding site for HP1 and also participate in silencing gene expression at euchromatic sites.
EpiSeeker Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) (ab115067) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K9 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 96 tests 8-Well Sample Strips (with Frame) 1 x 9 units 8-Well Standard Control Strips 1 x 3 units ESF1 (10X Wash Buffer) 1 x 20ml ESF2 (Antibody Buffer) 1 x 12ml ESF3 (Detection Antibody, 1 mg/ml) 1 x 10µl ESF4 (Fluoro Developer) 1 x 24µl ESF5 (Fluoro Enhancer) 1 x 24µl ESF6 (Fluoro Dilution) 1 x 8ml Standard Control (100 µg/ml) 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) images
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Functional Studies - EpiSeeker Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) (ab115067)ab115067 used to specifically measure global histone H3-K9 mono-, di-, and tri-methylation. The ratio of mono-, di-, and tri-methylated H3-K9 is proportional to the intensity of fluorescence. The absolute amount of the methylated H3-K9 can be quantitated by comparing to the standard control.
Protocols
References for EpiSeeker Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) (ab115067)
ab115067 has not yet been referenced specifically in any publications.
