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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. H3 (mono-methyl K9) is enriched in certain euchromatic domains, which have been postulated to be transcriptionally silent. H3 (di-methyl K9) and H3 (tri-methyl K9) mediate heterochromatin formation by forming a binding site for HP1 and also participate in silencing gene expression at euchromatic sites.
EpiSeeker Histone H3 (pan-methyl K9) Quantification Kit (Fluorometric) (ab115067) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3-K9 fluorometrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|8-Well Sample Strips (with Frame)||1 x 9 units|
|8-Well Standard Control Strips||Green Ringed||1 x 3 units|
|ESF1 (10X Wash Buffer)||1 x 20ml|
|ESF2 (Antibody Buffer)||1 x 12ml|
|ESF3 (Detection Antibody, 1 mg/ml)||1 x 10µl|
|ESF4 (Fluoro Developer)||1 x 24µl|
|ESF5 (Fluoro Enhancer)||1 x 24µl|
|ESF6 (Fluoro Dilution)||1 x 8ml|
|Standard Control (100 µg/ml)||1 x 20µl|
Our Abpromise guarantee covers the use of ab115067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115067 has not yet been referenced specifically in any publications.
Publishing research using ab115067 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"