EpiSeeker Histone H3 (phospho S28) Assay Kit (Fluorometric) (ab115130)
Overview
- Product nameEpiSeeker Histone H3 (phospho S28) Assay Kit (Fluorometric)See all Histone H3 (S28 Phosphorylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity0.5 ng/well
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Phosphorylation of histone H3 at serine 28 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. In vitro studies have shown that phosphorylation of histone H3 at both S10 and S28 is coupled with dynamic acetylation of H3, where histone H3 (phospho S28) had a higher steady state of acetylation than that of H3 (phospho S10). H3 (phospho S28) is regulated by the cell cycle and has been used as a mitotic marker.
EpiSeeker Histone H3 (phospho S28) Assay Kit (Fluorometric) (ab115130) allows the user to measure global histone H3 (phospho S28) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser28 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Notes
Store ESF3, ESF4 and the Standard Control at –20°C away from light.
Store all other components at 4°C away from light.
The components of the kit are stable for up to 6 months from the date of shipment, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8 well sample strips (with frame) 4 units 9 units 8 well standard control strips 2 units 3 units ESF1 (10X wash buffer) 1 x 10ml 1 x 20ml ESF2 (antibody buffer) 1 x 6ml 1 x 12ml ESF3 (detection antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (fluoro developer) 1 x 12µl 1 x 24µl ESF5 (fluoro enhancer) 1 x 12µl 1 x 24µl ESF6 (fluoro dilution) 1 x 4ml 1 x 8ml Standard control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (phospho S28) Assay Kit (Fluorometric) (ab115130)
ab115130 has not yet been referenced specifically in any publications.
