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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, try-methylation of histone H3 at lysine 27 (H3(try methyl K27)) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.
EpiSeeker Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045) allows the user to specifically measure histone H3-K27 tri-methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.
|Components||96 tests||2 x 96 tests|
|30% H2O2 Solution||1 x 0.5ml||1 x 1ml|
|8-Well Control Strips||2 units||4 units|
|ETM1 (10X Wash Buffer)||1 x 30ml||2 x 30ml|
|ETM2 (Permeabilizing Buffer)||1 x 30ml||2 x 30ml|
|ETM3 (Blocking Buffer)||1 x 20ml||2 x 20ml|
|ETM4 (Antibody Buffer)||1 x 10ml||1 x 20ml|
|ETM5 (Capture Antibody, 1000 µg/ml)||1 x 9µl||1 x 18µl|
|ETM6 (Detection Antibody, 200 µg/ml)||1 x 10µl||1 x 20µl|
|ETM7 (Developing Solution)||1 x 12ml||1 x 24ml|
|ETM8 (Stop Solution)||1 x 6ml||1 x 12ml|
|Microplate||1 unit||2 units|
|Tri-Methyl H3-K27 Control (20 µg/ml)||1 x 15µl||1 x 30µl|
Our Abpromise guarantee covers the use of ab115045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115045 has not yet been referenced specifically in any publications.
Publishing research using ab115045 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"