EpiSeeker Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045)
- Product nameEpiSeeker Histone H3 (tri methyl K27) Assay Kit (In Situ)See all Histone H3 (K27 methylation) kits ...
- Sample typeAdherent cells
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, try-methylation of histone H3 at lysine 27 (H3(try methyl K27)) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.
EpiSeeker Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045) allows the user to specifically measure histone H3-K27 tri-methylation in situ using cultured adherent cells in a quick and efficient procedure which can be finished within 3 hours.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 96 tests 2 x 96 tests 30% H2O2 Solution 1 x 0.5ml 1 x 1ml 8-Well Control Strips 2 units 4 units ETM1 (10X Wash Buffer) 1 x 30ml 2 x 30ml ETM2 (Permeabilizing Buffer) 1 x 30ml 2 x 30ml ETM3 (Blocking Buffer) 1 x 20ml 2 x 20ml ETM4 (Antibody Buffer) 1 x 10ml 1 x 20ml ETM5 (Capture Antibody, 1000 µg/ml) 1 x 9µl 1 x 18µl ETM6 (Detection Antibody, 200 µg/ml) 1 x 10µl 1 x 20µl ETM7 (Developing Solution) 1 x 12ml 1 x 24ml ETM8 (Stop Solution) 1 x 6ml 1 x 12ml Microplate 1 unit 2 units Tri-Methyl H3-K27 Control (20 µg/ml) 1 x 15µl 1 x 30µl
- Research Areas
Our Abpromise guarantee covers the use of ab115045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker Histone H3 (tri methyl K27) Assay Kit (In Situ) images
ab115045 detecting Histone H3 (tri methyl K27) in MDA-231 cancer cells. Cells were grown in microplate wells for 48 hours.
References for EpiSeeker Histone H3 (tri methyl K27) Assay Kit (In Situ) (ab115045)
ab115045 has not yet been referenced specifically in any publications.