EpiSeeker Histone H3 (tri methyl K27) Assay Kit (ab115051)
Overview
- Product nameEpiSeeker Histone H3 (tri methyl K27) Assay KitSee all Histone H3 (K27 methylation) kits ...
- Tests1 x 48 test
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, try-methylation of histone H3 at lysine 27 (H3(try methyl K27)) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.
EpiSeeker Histone H3 (tri methyl K27) Assay Kit (ab115051) is suitable for specifically measuring global histone H3-K27 tri-methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units EGT1 (10X Lysis Buffer) 1 x 5ml 1 x 10ml EGT10 (Stop Solution) 1 x 3ml 1 x 6ml EGT2 (Extraction Buffer) 1 x 8ml 1 x 16ml EGT3 (10X Wash Buffer) 1 x 14ml 1 x 28ml EGT4 (Histone Buffer) 1 x 0.5ml 1 x 1ml EGT5 (Blocking Buffer) 1 x 10ml 1 x 20ml EGT6 (Antibody Buffer) 1 x 6ml 1 x 12ml EGT7 (Capture Antibody, 100 µg/ml) 1 x 25µl 1 x 50µl EGT8 (Detection Antibody, 400 µg/ml) 1 x 10µl 1 x 20µl EGT9 (Developing Solution) 1 x 5ml 1 x 10ml Tri-Methyl H3-K27 Control (60 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H3 (tri methyl K27) Assay Kit (ab115051)
ab115051 has not yet been referenced specifically in any publications.
