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EpiSeeker Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric) (ab115072)

Q&A (4)

Overview

  • Product nameEpiSeeker Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric)See all Histone H3 (K27 methylation) kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, H3(try methyl K27) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.

    EpiSeeker Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric) (ab115072) allows the user to specifically measure global tri-methylation of histone H3-K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsFunctional Studies more details

Properties

Applications

Our Abpromise guarantee covers the use of ab115072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies FuncS

Protocols

References for EpiSeeker Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric) (ab115072)

ab115072 has not yet been referenced specifically in any publications.

Publishing research using ab115072 ? Please let us know so that we can cite the reference in this datasheet

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The credit was about ******* and this time, he has ******.
These kits were not satisfied again, he already purchased other vendor's products and doesn't need to use ab115070, ab115072 any more.
Please arrange credit note for our customer.

Sorry for the confusion.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: *****

As usual if you have any furth...

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Please arrange credit note for our customer.

A credit note has already been issued on the DD MM YYYY for these two kits. The reference of the credit note is ********.

In my previous email I said : "Would your customer agree to use their credit note to buy and try the kits for the 3rd ...

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As you know our customer is is havinga problem using you ab115070, ab115072.
He recently received new kits and tested again, but he could not any results eventhoug at standard.
So today I met him and checked his protocols, and there was no probl...

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Please find below the last email I received from the lab where the kits are produced :

"We are sorry to hear about it and not clear what caused it not work.
The procedure seems fine.
We did not receive any other complaint for the ...

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As you know our customer is is havinga problem using you ab115070, ab115072.
He recently received new kits and tested again, but he could not any results eventhoug at standard.
So today I met him and checked his protocols, and there was no probl...

Read More

Thank you for contacting us.

Thank you also for the detailed information about the use of the kits ab115070 and ab115072.

I do believe there is a problem with those kits, so please send our sincere apologies to the customer.

...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"