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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, H3(try methyl K27) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.
EpiSeeker Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric) (ab115072) allows the user to specifically measure global tri-methylation of histone H3-K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|8-Well Sample Strips (with frame)||4 units||9 units|
|8-Well Standard Control Strips||2 units||3 units|
|ESC1 (10X Wash Buffer)||1 x 10ml||1 x 20ml|
|ESC2 (Antibody Buffer)||1 x 6ml||1 x 12ml|
|ESC3 (Detection Antibody, 1 mg/ml)||1 x 5µl||1 x 10µl|
|ESC4 (Color Developer)||1 x 5ml||1 x 10ml|
|ESC5 (Stop Solution)||1 x 3ml||1 x 6ml|
|Standard Control (100 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115072 has not yet been referenced specifically in any publications.
Publishing research using ab115072 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"