EpiSeeker Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) (ab115057)
Overview
- Product nameEpiSeeker Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric)See all Histone H3 (K4 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. Enrichment of H3 (tri-methyl K4) is found at transcription start sites of active genes where they contribute to transcriptional initiation.
EpiSeeker Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) (ab115057) allows the user to specifically measure global tri-methylation of histone H3-K4 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESF1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESF2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESF3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (Fluoro-Developer) 1 x 12µl 1 x 24µl ESF5 (Fluoro-Enhancer) 1 x 12µl 1 x 24µl ESF6 (Fluoro-Dilution) 1 x 4ml 1 x 8ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
EpiSeeker Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) images
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Functional Studies - EpiSeeker Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) (ab115057)Histone extracts were prepared from MDA-231 cells using the EpiSeeker Histone Extraction Kit (ab113476) and the amount of trimethyl-H3-K4 was measured using ab115057.
Protocols
References for EpiSeeker Histone H3 (tri-methyl K4) Quantification Kit (Fluorometric) (ab115057)
ab115057 has not yet been referenced specifically in any publications.
