EpiSeeker Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) (ab115064)
- Product nameEpiSeeker Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric)See all Histone H3 (K9 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals
- Product overview
Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. In particular, H3(try methyl K9) mediates heterochromatin formation by forming a binding site for HP and is a stable heterochromatin mark which promotes gene silencing.is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing.
EpiSeeker Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) (ab115064) allows the user to specifically measure global tri-methylation of histone H3-K9 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESC1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESC2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESC3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESC4 (Color Developer) 1 x 5ml 1 x 10ml ESC5 (Stop Solution) 1 x 3ml 1 x 6ml Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl
- Research Areas
Our Abpromise guarantee covers the use of ab115064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) images
Histone extracts were prepared from MDA-MB-231 cells using the EpiSeeker Histone Extraction Kit (ab113476) and the amount of trimethyl-H3-K9 was measured using ab115064.
References for EpiSeeker Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) (ab115064)
ab115064 has not yet been referenced specifically in any publications.