EpiSeeker Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116)
Overview
- Product nameEpiSeeker Histone H4 (acetyl K5) Quantification Kit (Colorimetric)See all Histone H4 (K5 acetylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity2 ng/well
- Range20 ng/well - 5000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone H4 (acetyl K5) reflects the hyperacetylated stated in histone H4 and strongly correlates with active state of genes. H4 (acetyl K5) is also considered to be a marker of dexamethasone transactivation and a change in H4 (acetyl K5) is observed in cancer and inflammatory diseases.
EpiSeeker Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116) allows the experimenter to measure global acetylation of histone H4-K5 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H4-K5 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
- Notes
Upon receipt store ESC3 and Standard control at –20°C.
Store all other components at 4°C away from light. The kit is stable for up to 6 months from the shipment date, when stored properly.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units ESC1 (10X Wash Buffer) 1 x 10ml 1 x 20ml ESC2 (Antibody Buffer) 1 x 6ml 1 x 12ml ESC3 (Detection Antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESC4 (Color Developer) 1 x 5ml 1 x 10ml ESC5 (Stop Solution) 1 x 3ml 1 x 6ml Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 5µl 1 x 10µl Standard Control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116)
ab115116 has not yet been referenced specifically in any publications.
