EpiSeeker Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116)

Overview

  • Product nameEpiSeeker Histone H4 (acetyl K5) Quantification Kit (Colorimetric)See all Histone H4 (K5 acetylation) kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    2 ng/well
  • Range
    20 ng/well - 5000 ng/well
  • Assay time
    2h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone H4 (acetyl K5) reflects the hyperacetylated stated in histone H4 and strongly correlates with active state of genes. H4 (acetyl K5) is also considered to be a marker of dexamethasone transactivation and a change in H4 (acetyl K5) is observed in cancer and inflammatory diseases.

    EpiSeeker Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116) allows the experimenter to measure global acetylation of histone H4-K5 quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it is suitable for specifically measuring global histone H4-K5 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

  • Notes

    Upon receipt store ESC3 and Standard control at –20°C.

    Store all other components at 4°C away from light. The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115116 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker Histone H4 (acetyl K5) Quantification Kit (Colorimetric) (ab115116)

    ab115116 has not yet been referenced specifically in any publications.

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