EpiSeeker Histone H4 (di-methyl K20) Quantification Kit (Colorimetric) (ab115095)
Overview
- Product nameEpiSeeker Histone H4 (di-methyl K20) Quantification Kit (Colorimetric)See all Histone H4 (K20 methylation) kits ...
- Sensitivity5 ng/well
- Range20 ng/well - 2000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (di-methyl K20) has been described as repressive chromatin domain and involved in DNA damage response.
EpiSeeker Histone H4 (di-methyl K20) Quantification Kit (Colorimetric) (ab115095) enables the user to measure global di-methylation of histone H4-K20. The global H4-K20 di-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global di-methyl histone H4-K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115095 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8 well sample strips (with frame) 4 units 9 units 8 well standard control strips 2 units 3 units ESC1 (10X wash buffer) 1 x 10ml 1 x 20ml ESC2 (antibody buffer) 1 x 6ml 1 x 12ml ESC3 (detecting antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESC4 (color developer) 1 x 5ml 1 x 10ml ESC5 (stop solution) 1 x 3ml 1 x 6ml Signal enhancer 1 x 120µl 1 x 240µl Signal report solution 1 x 5µl 1 x 10µl Standard control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H4 (di-methyl K20) Quantification Kit (Colorimetric) (ab115095)
ab115095 has not yet been referenced specifically in any publications.
