For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (di-methyl K20) has been described as repressive chromatin domain and involved in DNA damage response.
EpiSeeker Histone H4 (di-methyl K20) Quantification Kit (Colorimetric) (ab115095) enables the user to measure global di-methylation of histone H4-K20. The global H4-K20 di-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global di-methyl histone H4-K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115095 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|8 well sample strips (with frame)||4 units||9 units|
|8 well standard control strips||Green Ringed||2 units||3 units|
|ESC1 (10X wash buffer)||1 x 10ml||1 x 20ml|
|ESC2 (antibody buffer)||1 x 6ml||1 x 12ml|
|ESC3 (detecting antibody, 1 mg/ml)||1 x 5µl||1 x 10µl|
|ESC4 (color developer)||1 x 5ml||1 x 10ml|
|ESC5 (stop solution)||1 x 3ml||1 x 6ml|
|Signal enhancer||1 x 120µl||1 x 240µl|
|Signal report solution||1 x 5µl||1 x 10µl|
|Standard control (100 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115095 has not yet been referenced specifically in any publications.
Publishing research using ab115095 ? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"