EpiSeeker Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094)
Overview
- Product nameEpiSeeker Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric)See all Histone H4 (K20 methylation) kits ...
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity1 ng/well
- Range10 ng/well - 2000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of the proper higher order structure of DNA and is consequently essential for chromosome condensation. It is also involved in gene silencing.
EpiSeeker Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094) enables the user to measure global mono-methylation of histone H4-K20. The global H4-K20 mono-methylation can also be changed by inhibition or activation of HMTs, making quantitative detection of global mono-methyl histone H4-K20 useful in developing a better understanding of epigenetic regulation of gene activation/repression and for developing HMT-targeted drugs. ab115094 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 48 tests 96 tests 8 well sample strips (with frame) 4 units 9 units 8 well standard control strips 2 units 3 units ESF1 (10X wash buffer) 1 x 10ml 1 x 20ml ESF2 (antibody buffer) 1 x 6ml 1 x 12ml ESF3 (detecting antibody, 1 mg/ml) 1 x 5µl 1 x 10µl ESF4 (fluoro developer) 1 x 12µl 1 x 24µl ESF5 (fluoro enhancer) 1 x 12µl 1 x 24µl ESF6 (fluoro dilution) 1 x 4ml 1 x 8ml Signal enhancer 1 x 120µl 1 x 240µl Signal report solution 1 x 5µl 1 x 10µl Standard control (100 µg/ml) 1 x 10µl 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094)
ab115094 has not yet been referenced specifically in any publications.
