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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of the proper higher order structure of DNA and is consequently essential for chromosome condensation. It is also involved in gene silencing.
EpiSeeker Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094) enables the user to measure global mono-methylation of histone H4-K20. The global H4-K20 mono-methylation can also be changed by inhibition or activation of HMTs, making quantitative detection of global mono-methyl histone H4-K20 useful in developing a better understanding of epigenetic regulation of gene activation/repression and for developing HMT-targeted drugs. ab115094 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
|Components||Identifier||48 tests||96 tests|
|8 well sample strips (with frame)||4 units||9 units|
|8 well standard control strips||Green Ringed||2 units||3 units|
|ESF1 (10X wash buffer)||1 x 10ml||1 x 20ml|
|ESF2 (antibody buffer)||1 x 6ml||1 x 12ml|
|ESF3 (detecting antibody, 1 mg/ml)||1 x 5µl||1 x 10µl|
|ESF4 (fluoro developer)||1 x 12µl||1 x 24µl|
|ESF5 (fluoro enhancer)||1 x 12µl||1 x 24µl|
|ESF6 (fluoro dilution)||1 x 4ml||1 x 8ml|
|Signal enhancer||1 x 120µl||1 x 240µl|
|Signal report solution||1 x 5µl||1 x 10µl|
|Standard control (100 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115094 has not yet been referenced specifically in any publications.
Publishing research using ab115094 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"