EpiSeeker Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100)
Overview
- Product nameEpiSeeker Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric)See all Histone H4 (K20 methylation) kits ...
- Tests1 x 96 test
- Sample typeTissue, Adherent cells, Suspension cells
- Sensitivity1 ng/well
- Range10 ng/well - 2000 ng/well
- Assay time2h 30m
- Species reactivityReacts with: Mouse, Human
Predicted to work with: all Mammals - Product overview
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of proper higher order structure of DNA and is consequently essential for chromosome condensation, as well as functioning in gene silencing. H4 (di-methyl K20) has been described as another repressive chromatin domain and is involved in the DNA damage response. H4 (tri-methyl K20) acts as a passive feature or structure determinant for chromatin degradation and release, as well as being an epigenetic marker of early apoptosis. H4 (tri-methyl K20) is also considered as a common hallmark of human cancer.
EpiSeeker Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100) enables the user to measure global mono-, di- and tri-methylation of histone H4-K20. The global H4-K20 pan-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global pan-methyl histone H4-K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115100 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsPlease refer to protocols.
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Components 96 tests 8 well sample strips (with frame) 1 x 9 units 8 well standard control strips 1 x 3 units ESF1 (10X wash buffer) 1 x 20ml ESF2 (antibody buffer) 1 x 12ml ESF3 (detecting antibody, 1 mg/ml) 1 x 10µl ESF4 (fluoro developer) 1 x 24µl ESF5 (fluoro enhancer) 1 x 24µl ESF6 (fluoro dilution) 1 x 8ml Signal enhancer 1 x 240µl Signal report solution 1 x 10µl Standard control (100 µg/ml) 1 x 20µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab115100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for EpiSeeker Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100)
ab115100 has not yet been referenced specifically in any publications.
