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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of proper higher order structure of DNA and is consequently essential for chromosome condensation, as well as functioning in gene silencing. H4 (di-methyl K20) has been described as another repressive chromatin domain and is involved in the DNA damage response. H4 (tri-methyl K20) acts as a passive feature or structure determinant for chromatin degradation and release, as well as being an epigenetic marker of early apoptosis. H4 (tri-methyl K20) is also considered as a common hallmark of human cancer.
EpiSeeker Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100) enables the user to measure global mono-, di- and tri-methylation of histone H4-K20. The global H4-K20 pan-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global pan-methyl histone H4-K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115100 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
|8 well sample strips (with frame)||1 x 9 units|
|8 well standard control strips||1 x 3 units|
|ESF1 (10X wash buffer)||1 x 20ml|
|ESF2 (antibody buffer)||1 x 12ml|
|ESF3 (detecting antibody, 1 mg/ml)||1 x 10µl|
|ESF4 (fluoro developer)||1 x 24µl|
|ESF5 (fluoro enhancer)||1 x 24µl|
|ESF6 (fluoro dilution)||1 x 8ml|
|Signal enhancer||1 x 240µl|
|Signal report solution||1 x 10µl|
|Standard control (100 µg/ml)||1 x 20µl|
Our Abpromise guarantee covers the use of ab115100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115100 has not yet been referenced specifically in any publications.
Publishing research using ab115100 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"